Re: Live histograms and overlays

Posted by Nagesh Adluru on Sep 22, 2015; 12:05pm
URL: http://imagej.273.s1.nabble.com/Re-Live-histograms-and-overlays-tp5014403.html

nadluru <http://forum.imagej.net/users/nadluru>
September 22

Dear ImageJ community,

I posted this request on the new forum that Curtis Rueden mentioned but am
posting it also here in the hopes I can get help quicker.

Could you kindly help me with the following tasks

*1) Live histogram of "Z axis profile":* I have a hyperstack of images and
I would like to see "live histograms" of the Z-axis profiles. I can plot
the profiles but would like to see their histograms and preferably live.
The standard histogram command in the Analyze menu does this for line
profiles but not for the Z-axis profiles.

*2) Overlays of images with different LUTs and thresholds and transparency:* I
have statistical maps and underlying brain images. I would like to
threshold the statistical maps and use some colorful LUTs and overlay them
on the brain images (which themselves are in grayscale) so that the zero
values in the statistical maps are transparent. Once overlaid I am hoping I
can use "magic montage" to generate various presentations from it. I have
the magic montage macro working but the getting the right overlays has been
really challenging.

Thanks so much!
Nagesh

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