Posted by
Yisong Zhen on
Sep 30, 2015; 7:41am
URL: http://imagej.273.s1.nabble.com/how-to-measure-the-volume-of-the-tissue-tp5014496p5014499.html
Dear Romain,
The mouse heart is so small and wet (with fixation buffer), and I am afraid
that system error would not allow me to accurately get the
volume information by Archimedes Principle. At least I have no
precision-proof equipment to measure the water volume. This might be a way
but it take time anyway. As your first proposal, if I understand correctly,
it need sample sliced. And this is not permitted. My question is: I have
digital camera and tiny mouse heart, I want to take photos in several
positions of the heart under microscopy. From those digital information, I
want to get the volume of the mouse heart. Is there some plugin can do it?
Anyway, thanks again your suggestions.
Yisong
On Wed, Sep 30, 2015 at 3:16 PM, Romain Guiet <
[hidden email]> wrote:
> Hi YiSong,
> you may have a look to stereology technics and the cavalieri estimator :
>
http://www.stereology.info/cavalieri-estimator/.
> or you can try to measure the volume displacement to calculate the volume,
> using Archimedes Principle.
> Cheers,
> Romain
>
>
> ---------------------------------------------------------------
> Dr. Romain Guiet
> Bioimaging and Optics Platform (PT-BIOP)
> Ecole Polytechnique Fédérale de Lausanne (EPFL)
> Faculty of Life Sciences
> Station 19, AI 0140
> CH-1015 Lausanne
>
> Phone: [+4121 69] 39629
>
http://biop.epfl.ch/> ---------------------------------------------------------------
>
> ________________________________________
> De : ImageJ Interest Group [
[hidden email]] de la part de Yisong
> Zhen [
[hidden email]]
> Envoyé : mercredi 30 septembre 2015 07:45
> À :
[hidden email]
> Objet : how to measure the volume of the tissue
>
> Dear ImageJ users,
>
> I want to measure the volume of the each mouse heart. I want to use digital
> camera to take the photo of the tissue and extract the essential
> information to calculate the volume. How can I do and use which kind of
> plugin to complete my task? Thanks.
>
> --
>
> //---------------------------------------------------------------------------------------
> // We have a hunger of the mind which asks for knowledge
> // of all around us, and the more we gain, the more is
> // our desire; the more we see, the more we are capable
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> @
> YiSong Zhen, Ph.D
> Sino-German Laboratory for Molecular Medicine
> Fu Wai Hospital
> Beilishilu 167, 100037 Beijing
> P.R.China
>
http://www.cardiosignal.org> Tel: 86-10-88398058
>
> @----------------------------------------------------------------------------------------
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