Re: Compare Images to Quantify Cleaning Performance

Posted by Peter Haub on Oct 01, 2015; 9:22am
URL: http://imagej.273.s1.nabble.com/Compare-Images-to-Quantify-Cleaning-Performance-tp5014503p5014515.html

Hi Frank,

ensure that the images have identical scale and align both images via
phase correlation. Then it should be possible to detect 'the' image
differences in the overlapping region of both images by 'Image
Calculator/ Difference'. ('The' differences means at least areas where
objects are vanished etc.  )
Take care of image intensity, color and contrast. Image differences on
that level should not originate from unstable illumination conditions or
varying camera settings.

In case of different image scales you have to map the images in relation
to reference points (which can be either selected manually or found
automatically e.g. by techniques like SIFT).

Regards
Peter

On 01.10.2015 10:11, Frank Robson wrote:

> Hello everyone,
>
> thank you for the answers.
> Here are some sample images of the test plates before and after the cleaning
> process:
> <http://imagej.1557.x6.nabble.com/file/n5014514/V6vz.jpg>
> <http://imagej.1557.x6.nabble.com/file/n5014514/V6n1z.jpg>
> The distance between plate and camera is always kept the same, however the
> angle might differ. I built a kind of blackbox so that no external lighting
> will infer (the camera is always kept in manual mode).
> I'll have a look if it would be possible to use a tripod in order to assure
> a constant angle for the camera.
>
> Cheers,
> Aaron
>
>
>
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>
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