Measuring distance between points for tree ring widths

Posted by lindbeem on Oct 15, 2015; 3:30am
URL: http://imagej.273.s1.nabble.com/Measuring-distance-between-points-for-tree-ring-widths-tp5014639.html

I have multiple tree core .tiff files that I want to analyze and calculate the distance from the pith to each respected ring.  I have used the multiple point tool and have marked the edge of each ring starting from the most recent year's growth all the way to marking the middle of the pith.  I'm planning to label them by calendar year and use the distances to do further calculations.  I am new to using ImageJ and macros, but essentially, I would like to be able to have a script calculate the distance between the tree ring to the pith for each measurement using the x and y coordinates.  Images were scanned using 3200 DPI resolution which is already set.  (I.E. If the tree is 82 years old, there will be 83 points.  Point #83 will be at the center of the core (pith), and every other point will mark the end of a tree ring.  So I want to measure the distance from point 1 to 83, 2 to 83, 3 to 83, 4 to 83, etc.)

Is there a tool that allows you to enter in the coordinates and specify how you would like them measured?   Also, I have marked the points on the tree core, but I am unable to see them in the ROI manager.  Is there an extra step that I need to do in order to get those points to show up?

Any help will be appreciated.  Thank you in advance.