Re: Grid/Collection Stitch
Posted by
Majurski, Michael Paul (Fed) on
Oct 19, 2015; 4:30pm
URL: http://imagej.273.s1.nabble.com/Grid-Collection-Stitch-tp5014667p5014681.html
Cheuk,
In the past when I have had trouble getting the grid/collection stitching plugin to assemble a satisfactory image I have set the regression threshold very low (0.1). I have found that if you provide a correct image overlap (that is also uniform between all images) the plugin will sometimes generate a correct image.
~Michael Majurski
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Cheuk Tam
Sent: Friday, October 16, 2015 1:53 PM
To:
[hidden email]
Subject: Grid/Collection Stitch
Hi All,
I'm new to FIJI, and I was troubleshooting the Grid stitching plugin. It was able to produce a stitched image, but some areas are aligned incorrectly. For example, the nuclei from overlapping constituent images are juxtaposed in the fused image instead of merged together (upper right part of the neurosphere, adjacent to the 2 bubbles).
The images are in the following folder:
https://www.dropbox.com/sh/8idzyd0rh3qt3in/AADUA93Kd-31bK-pzzwCgt50a?dl=0"C3-08C6 _A_comp 10x Fused" is the stitched image
"08C6 _A_Hoechst_10x_13" is the corresponding original
I've tried to increase the regression threshold and decrease the displacement threshold, but without any luck. Is there anything I can change in the way I take the pictures or stitch them together? Is there such a thing as too much overlap in the individual pictures?
Thank you for your help.
Sincerely,
Cheuk
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