Re: Grid/Collection Stitch

Posted by Cheuk Tam-2 on Oct 21, 2015; 6:39pm
URL: http://imagej.273.s1.nabble.com/Grid-Collection-Stitch-tp5014667p5014706.html

Dear Dr. Majurski,

Thank you for your suggestions.  Lowering the regression threshold gave better alignment to parts of the image.  Unfortunately, the nuclei were still misaligned.  I was able to have proper alignment when stitching the channels (red for Beta-3 Tubulin and blue for Hoechst) separately.  However, it was difficult merge the two channels together after stitching because the fused images are of different sizes.  I was wondering there is an issue with registering different channels together.  If so, will converting the channels to timepoints (perhaps by designating channels as tandem timepoints) help? Is there a way to do this in MIST?  Thank you for your help.

Sincerely,
Cheuk

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