Posted by
Menelaos Symeonides on
Oct 22, 2015; 3:19pm
URL: http://imagej.273.s1.nabble.com/Multi-measure-with-moving-ROI-tp5014723p5014724.html
Hi Kirk,
I don't have a good answer for you, just throwing my hat in because I've
just been dealing with a very similar issue and would be interested to
hear what's out there. Because I had to get this done and couldn't wait
for a better solution to come up, I ended up doing the following:
1. Under Analyze - Set Measurements, I selected the measurements I was
interested in.
2. Under Analyze - Tools, I opened ROI Manager.
3. In each frame, I selected my ROI (in my case, a T cell I was tracking
in the brightfield channel to look for fluorescence appearing in the GFP
channel), making sure I had my channel of interest selected, and after
selecting each ROI I hit "t" to add the ROI to the ROI manager, and
advanced to the next timepoint.
4. After saving each ROI in each frame, I saved my set of ROIs within
ROI Manager, and then hit Measure under ROI Manager, which spit out a
nice table of my results.
Extremely time consuming, and after the 1500th ROI, rather mind-bending,
but I got my data.
Good luck,
Mel
On 10/22/2015 10:58 AM, Kirk_Taylor wrote:
> Hi,
> Firstly, apologies if there is already a thread for this, but I couldn't
> find one.
>
> In my experiments I am measuring intracellular fluorescence of an
> ion-sensitive dye in response to stimulation by an agonist. These
> experiments are over a 10 minute time course with an image acquired every
> second (600 frames in total). I have used multi measure to assess the change
> in fluorescence and this works quite well on the whole (thank you ImageJ!!).
> However, my cells are non-adherent and during the course of the experiment
> some cells start to drift (slowly). This causes problems as some ROI's are
> no longer placed appropriately by the end of the of the experiment. Is there
> a way to get ImageJ to reposition the ROI for each frame?
>
> Many thanks,
> Kirk
>
>
>
> --
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>
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Menelaos Symeonides
University of Vermont
Cell & Molecular Biology Graduate Program
Department of Microbiology and Molecular Genetics
318 Stafford Hall
95 Carrigan Dr
Burlington, VT 05405
[hidden email]
Phone: 802-656-1161
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