http://imagej.273.s1.nabble.com/help-with-3D-segmentation-tp5014798p5014800.html
> On Nov 1, 2015, at 08:06 , Aryeh Weiss <
[hidden email]> wrote:
>
> I have been asked to do a 3D segmentation of a DAPI stained image, such as the image stack available at:
>
https://drive.google.com/open?id=0B9hvIdSL8kGyY0NiRzRkaXlINmM>
> I find that I can do very well in 2D by blurring the image and doing peak detection, or gray-scale watershed.
> Gabriel Landini's domes followed by a peak detection or thresholding step also works nicely.
> However, I have not succeeded in extending this to 3D, using the 3D morphological segmentation tools.
>
> A 3D-domes would be nice, but I have not found that.
> I tried peak detection or 2D-domes and then 3D object counting, but there is still too much overlap in the Z-direction, so that nuclei which
> overlap vertically are counted as one object.
>
> So I seek to tap into the knowledge available on this list, because I am sure someone has already done this type of segmentation.
>
> Thanks in advance
> --aryeh
>
> --
> Aryeh Weiss
> Faculty of Engineering
> Bar Ilan University
> Ramat Gan 52900 Israel
>
> Ph: 972-3-5317638
> FAX: 972-3-7384051
>
>
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