Nuclei Outline from DAPI pictures

Posted by biscmm22 on Nov 01, 2015; 6:43pm
URL: http://imagej.273.s1.nabble.com/Nuclei-Outline-from-DAPI-pictures-tp5014805.html

Hi there,

I am a student working with HeLa cells.  I am using ImageJ to measure the difference in fluorescence in cell nuclei between different treatment groups.  My Dapi pictures are clear, and the nuclei are visible.  The location of the nucleus is less clear in the other pictures of protein (Ran, Topoisomerase, etc).  I am trying to find a way to outline the nuclei on the dapi pictures and then apply those outlines to the protein pictures.  I have tried multiple ways, but I can't seem to get it to work.  Is there a way that I could do this?

Thanks in advance for your help!

Sincerely,
Melissa