Posted by
Glen MacDonald-2 on
Nov 05, 2015; 4:42pm
URL: http://imagej.273.s1.nabble.com/Problem-with-channel-bleed-through-after-splitting-channels-from-RGB-image-tp5014842p5014862.html
Does your Evos have the monochrome camera or the RGB? The Bayer mask on a single chip RGB camera has a lot of overlap between green and blue, so the green detectors always will detect a little bit of the blue, even if its leaking through an the LED module's emission filter. Bayer mask images are OK for qualitative use, some morphometry is possible after channel subtraction, but useless for intensity based quantitation. The Bayer masks also overlap green and red.
Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Cellular Morphology Core
Center on Human Development and Disability
Box 357923
University of Washington
Seattle, WA 98195-7923 USA
(206) 616-4156
[hidden email]
On Nov 5, 2015, at 7:18 AM, FrankyG <
[hidden email]> wrote:
> Dr. Sheffield,
>
> Our scope is a bit different in that for each channel a different light
> source is used. It still filters the light a bit but the DAPI light source
> is different than the GFP light source. Unless I understood you wrong and
> you were not saying that the cubes all filter from one main light source.
>
> I will try your method of saving and then merging the files again. Though it
> requires more macros I'm all for efficient workflow so it will get done
> somehow.
>
> Dr. Swayne,
>
> I will check with the EVOS people if it is possible to change the LUT used.
> If it is possible and that solves the problem than you have many thanks from
> me for saving me from a lot of extra programming.
>
>
> Thanks for the answers, it's time to get back to work! I will post if I have
> further issues.
>
>
>
> --
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