Re: Problem with channel bleed-through after splitting channels from RGB image
Posted by FrankyG on Nov 05, 2015; 9:34pm
URL: http://imagej.273.s1.nabble.com/Problem-with-channel-bleed-through-after-splitting-channels-from-RGB-image-tp5014842p5014867.html
Dr. Landini,
I save all of them in .tif.
I'm not sure what artifacts you are referring to and I stopped using the RGB for quantitative analysis because of the issues I realized once I split the images. Saving them as separate channels fixes almost all the issues I have been having. Perhaps look at the images that are separate channels and see if you can find artifacts? Those are the ones I am using now, not the RGB.
To be clear they are saved separately from the RGB, perhaps that was not clear.
Dr. MacDonald,
Yes our EVOS has a monochrome camera and the representative explained it to me.
So are you saying that even if I save the channels separately that the imaging system I am using is absolutely useless for quantitative studies? If so I'll have to notify my PI ASAP because I do not think he was aware of that.