Re: Help begineer to detect cells in petri dish

Posted by Rasband, Wayne (NIH/NIMH) [E] on Nov 10, 2015; 5:25pm
URL: http://imagej.273.s1.nabble.com/Help-begineer-to-detect-cells-in-petri-dish-tp5014903p5014905.html

> On Nov 10, 2015, at 9:59 AM, MariaS <[hidden email]> wrote:
>
> <http://imagej.1557.x6.nabble.com/file/n5014903/petri_dish.jpg>
>
> Hello everybody!
> I just started using ImageJ but I find it pretty hard to get the results I
> want.
> I have for example this image from a petri dish with a filter with bacteria
> and I want to count the number of the cells. First I draw a circle to choose
> my area of interest, then i select Clear Outside and then I try with some
> filters and by adjusting the threshold to detect my cells. But the stripes
> of the filter on the petri dish create problems and I cannot find a way to
> remove them. Does anyone have some ideas that might help?

Why not count manually? It only took me a couple of minutes to count the cells (86) using ImageJ's multi-point tool.

    http://wsr.imagej.net/images/petri_dish2.jpg

-wayne

> --
> View this message in context: http://imagej.1557.x6.nabble.com/Help-begineer-to-detect-cells-in-petri-dish-tp5014903.html
> Sent from the ImageJ mailing list archive at Nabble.com.

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html