Re: Help begineer to detect cells in petri dish

Posted by Herbie on Nov 10, 2015; 7:00pm
URL: http://imagej.273.s1.nabble.com/Help-begineer-to-detect-cells-in-petri-dish-tp5014903p5014910.html

Dear Maria,

you wrote:
"I did not think that it would be so hard to count the cells with ImageJ."

Did you ever consider that the problem is with your image(s) and not
with ImageJ?

The image you've provided shows rather low spatial resolution, lots of
JEPG-artifacts, circular reflections, a high contrast grid, and
partially overlapping cells.

There is little chance to count the cells automatically and reliably, no
matter what software you use.

Best

Herbie

::::::::::::::::::::::::::::::::::::
Am 10.11.15 um 19:00 schrieb MariaS:
> Thanks for your answer!
>
> The thing is that I have many images of this kind and in the end we
> want to create a Macro that could automatically count the cells. So
> the first step is at least to understand how to set the settings
> right manually. I did not think that it would be so hard to count the
> cells with ImageJ. And I started reading the guide of ImageJ but it
> is not really helpful in this case.

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