Posted by
Joel Sheffield on
Nov 10, 2015; 6:47pm
URL: http://imagej.273.s1.nabble.com/Help-begineer-to-detect-cells-in-petri-dish-tp5014903p5014911.html
Hi Maria,
It may be possible, with your image, to threshold the particles, and use
the discrimination functions of "Analyze Particles" to count only objects
of a given size range and/or circularity. Part of the problem in that case
is that you have several examples of overlapping colonies. In that case,
you could see if the watershed procedure would work around the grid lines.
Another issue is that your image is taken at quite low pixel density so
that discrimination is even more difficult.
That said, you might want to calculate the time that it would take to set
up an automatic system and set it against Wayne's suggestion. I suppose it
depends on what you mean by "many".
Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail:
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On Tue, Nov 10, 2015 at 1:00 PM, MariaS <
[hidden email]> wrote:
> Thanks for your answer!
>
> The thing is that I have many images of this kind and in the end we want to
> create a Macro that could automatically count the cells. So the first step
> is at least to understand how to set the settings right manually. I did not
> think that it would be so hard to count the cells with ImageJ. And I
> started
> reading the guide of ImageJ but it is not really helpful in this case..
>
>
>
> --
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