Overlay troubles with AnalyzeParticles

Posted by Laurent.guerard on Nov 24, 2015; 8:16am
URL: http://imagej.273.s1.nabble.com/Overlay-troubles-with-AnalyzeParticles-tp5015012.html

Hi,

I'm writing a macro to detect and measure green and red area in images and I am using the Analyze Particles plugin to do so.

However, I would like to save the overlay, the detected "particles", in the image which I have succeded. But when examining the output from this plugin, I can see that the measurements are ok but the region shown by the overlay are not.

What I mean is that, for example, it doesn't take into consideration the holes in an object and only show the outlines of the objects even though I ask for the overlay.

Any ideas ? I tried with this "finding edges" plugin but it doesn't filter out small objects like Analyze Particles..

Thanks !