measuring the running width of a kymograph 'object'

Posted by TimFeinstein on Nov 30, 2015; 9:12pm
URL: http://imagej.273.s1.nabble.com/measuring-the-running-width-of-a-kymograph-object-tp5015052.html

Hello,

I have a kymograph of a transect through beating heart muscle measured
using light sheet fluorescence.  I would like to graph 'strain' and
'strain rate' over time, reflecting the distance and rate of movement of
the inner wall of the ventricle of interest.  That would require measuring
the horizontal dimension of the inner thresholded 'object' in the attached
kymograph from top to bottom in 500 steps.  Does anyone know of a way to
do this in ImageJ/Fiji?  Apologies if this has come up before and I just
failed to find it.

Many thanks,


Tim

Timothy Feinstein, Ph.D.
Research Scientist
University of Pittsburgh Department of Developmental Biology


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