Posted by
Cammer, Michael on
Dec 01, 2015; 2:19am
URL: http://imagej.273.s1.nabble.com/measuring-the-running-width-of-a-kymograph-object-tp5015052p5015056.html
Except for a few stray pixels, this may solve the problem.
// The next two steps fill in the missing pixels on the left side of the kymogram
makeRectangle(112, 52, 12, 2);
run("Set...", "value=0");
// Here is the macro which only works if the left and right waves are solid
// Counts pixels of value 255 in single pixel high box from top to bottom of image
w = getWidth();
doWand(1,1);
run("Set...", "value=0");
doWand(w-1, 1);
run("Set...", "value=0");
print("\n" + getTitle());
for (i=0; i<getHeight; i++) {
makeRectangle(0,i, w,1);
getHistogram(values, counts, 256);
print(counts[255]);
} // for i
_________________________________________
Michael Cammer, Optical Microscopy Specialist
http://ocs.med.nyu.edu/microscopyhttp://microscopynotes.com/Cell: (914) 309-3270
________________________________________
From: ImageJ Interest Group [
[hidden email]] on behalf of Feinstein, Timothy N [
[hidden email]]
Sent: Monday, November 30, 2015 4:19 PM
To:
[hidden email]
Subject: measuring the running width of a kymograph 'object'
Hello,
I have a kymograph of a transect through beating heart muscle measured
using light sheet fluorescence. I would like to graph 'strain' and
'strain rate' over time, reflecting the distance and rate of movement of
the inner wall of the ventricle of interest. That would require measuring
the horizontal dimension of the inner thresholded 'object' in the attached
kymograph from top to bottom in 500 steps. Does anyone know of a way to
do this in ImageJ/Fiji? Apologies if this has come up before and I just
failed to find it.
Many thanks,
Tim
Timothy Feinstein, Ph.D.
Research Scientist
University of Pittsburgh Department of Developmental Biology
--
ImageJ mailing list:
http://imagej.nih.gov/ij/list.html------------------------------------------------------------
This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
=================================
--
ImageJ mailing list:
http://imagej.nih.gov/ij/list.html