Temporal high pass filter within ImageJ

Posted by CARL Philippe (LBP) on Dec 03, 2015; 9:52am
URL: http://imagej.273.s1.nabble.com/Temporal-high-pass-filter-within-ImageJ-tp5015115.html

Dear all,

I try to perform fluorescence spectroscopy experiences where I acquire
several (typically 100) pictures for which I determine for each pixel the
intensity SD to average ratio in order to extract the relative brightness
distribution over the picture.

Nevertheless during these temporal acquisitions, the fluorophores are
subjected to photo bleaching which effect needs to be "cleaned away" from
the calcultation.

My idea for correcting this effect is to implement a moving average filter
pixel by pixel over the time series pictures.

Where I would determine the temporal average (over a certain number of
pictures to be determined empirically) which I would then subtract from the
given pixel value over the whole stack.

But maybe somebody has a better idea and/or already a plugin/macro for
performing such a correction.

I thank you very much in advance for your help and hints.

My best regards,

Philippe

 

Philippe CARL

Laboratoire de Biophotonique et Pharmacologie

UMR 7213 CNRS - Université de Strasbourg

Faculté de Pharmacie

74 route du Rhin

67401 ILLKIRCH

Tel : [phone]

 


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