Re: thresholding and detection issues when analyzing particles to obtain object counts in a petri dish

Posted by ben_king on Dec 08, 2015; 9:35pm
URL: http://imagej.273.s1.nabble.com/thresholding-and-detection-issues-when-analyzing-particles-to-obtain-object-counts-in-a-petri-dish-tp5015178p5015179.html

Rich,

Try Process > Find Maxima. It's like a watershed separation, but for non-binary images. I got a count of 3050 for your image, and looks like most spots were identified. Playing with the noise tolerance might improve performance further.