Re: thresholding and detection issues when analyzing particles to obtain object counts in a petri dish

Posted by Bethany Uttaro on Dec 10, 2015; 7:10pm
URL: http://imagej.273.s1.nabble.com/thresholding-and-detection-issues-when-analyzing-particles-to-obtain-object-counts-in-a-petri-dish-tp5015178p5015209.html

Hi Rich,

Have you tried photographing the dish instead of scanning it? Use a digital camera with an option for RAW images, and if you are shooting close up or in macro, check for distortion using a grid. The distortion isn't a problem for counting, of course, but will cause problems if the same images are later used for dimensions. Oh, and include a scale/ruler in the image, of course.

Bethany

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