Posted by
Jeff Spector-2 on
URL: http://imagej.273.s1.nabble.com/grid-aligner-plugin-error-tp5015194p5015219.html
On Fri, Dec 11, 2015 at 12:24 AM, Nico Stuurman <
[hidden email]>
wrote:
> Hi Jeff,
>
> On 12/10/15 2:29 PM, Jeff Spector wrote:
>
>> I've got grid aligner working now ( I just removed it and reinstalled it
>> under FIJI and it works now.
>> The alignment between my images looks perfect, but it looks like the
>> plugin
>> is changing my intensity values.
>> Is this normal? as an example I drew a line in the raw data and the
>> aligned data in the aligned data the min and max are 0 and 257 where as in
>> the original raw data they are 250.69 and 386.89
>>
>
> Looks like you are working with images of type float (32-bit images). I
> never tried those, and certainly did not provide a code-path for them, so
> it is unclear what will happen. You could try converting first to 16-bit
> images.
>
>
>
>
> Best,
>
> Nico
>
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> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html>
Hi,
The images I'm using are 16-bit (they come from an andor ixon camera) What
I am seeing is that after calculating the transform when I apply it the new
image is registered fine, but when I look at the image histograms, the
histogram for channel 2 is always a series of discrete spikes, where as in
the original image it was a smooth looking histogram. The histogram for
channel 1 remains unchanged. Please let me know what you think..
-Jeff
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