Posted by
Michael Schmid on
Jan 03, 2016; 4:03pm
URL: http://imagej.273.s1.nabble.com/plugin-for-crowded-field-photometry-fitting-overlapping-identical-objects-tp5015311.html
Hi everyone,
is there some out there who knows about an ImageJ plugin that does roughly
what astronomers call "crowded-field photometry"?
I.e., imagine that you have stars that appear slightly blurred, and the
images of the individual stars are overlapping. Apart form different
brightness, the stars are identical (identical point spread function PSF).
In super-resolution microscopy one might encounter the same problem if
there is an overlap of the image of neighboring fluorescent particles.
Such images cloud be analyzed by deconvolution, but this has some
limitations. I am looking for a plugin that tries to fit the image with
the sum of a large number of PSF functions (preferably with subpixel
positions). Probably 2D Gaussians would be sufficient.
If someone is aware of an easy-to-understand algorithm or code that could
be converted into an ImageJ plugin, such hints would be also helpful.
[For the astronomers among you: yes, I am aware that there is quite some
literature on crowded-field photometry, but delving into it and converting
one of these methods into code is beyond what I could do within reasonable
time.]
Thank you,
Michael
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