Re: Distance from fixed object to threshold particle in a stack

Posted by burt46 on Jan 04, 2016; 12:23pm
URL: http://imagej.273.s1.nabble.com/Distance-from-fixed-object-to-threshold-particle-in-a-stack-tp5015307p5015316.html

Thanks Jeremy

I tried the Distance Map approach but have distances over 255 pixels. I did the analyse particles for the pixel positions too but could not get the shortest distance between the two ROIs. Both my ROIs are perimeter analysis and not centroid.

The nearest I came to a solution was the graph plugin demo>

http://rsb.info.nih.gov/ij/plugins/graph/index.html

However, this wont work in stacks.

The following also worked well for stacks, but is centroid to perimeter and not perimeter to perimeter:

http://microscopynotes.com/imagej/shortest_distance_to_line/index.html