http://imagej.273.s1.nabble.com/Need-help-with-some-weird-Area-calculaitons-tp5015328p5015329.html
measurement for lots of fibers at once (i.e. everything visible in your
> Hello,
> I was wondering if the following is possible using image J:
>
> I have pictures of some fibers before and after they have been coated with
> a thick polymer film. The pictures are taken at the same magnification and
> correspond to the same area, however after applying the film the fibers
> tilt a bit so the images don't exactly superimpose. I am interested in
> calculating the areas occupied by the fibers so I can compare the before
> and after coating values.
> I am trying to make a quick assessment of the increase in thickness due to
> the coating so of course I know I could measure fiber by fiber but that is
> not the point since I have to evaluate many coatings and do not need a very
> precise value, it is more for comparison purposes. Any ideas if something
> of this sort can be done?, how?
> I've tried subtracting images but that gives me another image when I need
> a numeric value plus the tilting of the fibers makes it difficult to even
> say anything from the resulting image. I have binarized the images and
> tried to convert one in background or mask to subtract from the "after"
> image but have the same issue with the tilted fibers. Then I ran out of my
> ImageJ knowledge so as you can see it is very limited.
> Any help is really appreciated.
> Alejandra
>
> --
> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html>