cross sectional area

Posted by Sarah Mohamed on Jan 06, 2016; 5:10am
URL: http://imagej.273.s1.nabble.com/cross-sectional-area-tp5015330.html


Hi,
I wonder if you can kindly answer my question about measuring cross-sectional area of cells using image j.
i have images with scale bar 50 um (X20)  and scale bar 20 um (X40) but i dont have a pic of a ruler at 2 magnification power
can i set set the scale bar in image J by using the pic scale bar as the known distance and change the scale for each magnification power?
will i be able to compare  results of both CSA?
Also, will the resulted area be in square micrometer?
Thanks in advance.
Kind Regards,
Sarah M. Mosaad, M.Sc.
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