How to use line selection and count the number of specific colored pixels on either sides of the line?

Posted by ram prasad on Jan 08, 2016; 3:03pm
URL: http://imagej.273.s1.nabble.com/How-to-use-line-selection-and-count-the-number-of-specific-colored-pixels-on-either-sides-of-the-lin-tp5015347.html

Dear All,

I would like to know how to do the following in imageJ.

I'm trying to get the number of yellow and black coloured pixels on either
sides of a line, which will be drawn by me using the line selection tool.
I'm attaching one such image wherein I have drawn the line for your
reference.

I know how to how use the thresholder to get at the counts of yellow and
black pixels in the whole image but I don't know how to go about this. I
googled to see if anyone else had done something similar but the examples I
found usually involved splitting the whole image into two equal halves
based on the image dimensions. I don't think it is possible in my case as
the coordinates of the line cannot be deduced from the image dimensions.

I'd really appreciate it if you could let me know how to go about this or
if you can suggest a different approach.

Let me know if you need any more details from my side.

Thank you very much.

Cheers,
Ram

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