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Re: Mitochondrial analysis on z-stack

Posted by Василий Попков on Jan 15, 2016; 10:18am
URL: http://imagej.273.s1.nabble.com/Mitochondrial-analysis-on-z-stack-tp5015379p5015388.html

Thank you a lot. Now I know where to look. I'm a little ashamed, because
now it seems obvious... But thanks :)

2016-01-14 17:59 GMT+03:00 Jason Miller <[hidden email]>:

> Hi Visaly-
>
> I'm sure Thomas Bouldier will chime in here, but his 3D analysis suite
> contains a range of options to do exactly what you are asking.
>
> http://imagejdocu.tudor.lu/doku.php?id=plugin:stacks:3d_ij_suite:start
>
> For simpler 3d segmentation, you can also use the built-in 3D objects
> counter function:
>
> http://fiji.sc/3D_Objects_Counter
>
> There are a huge range of other plugins out there that will count particles
> in 3D (e.g. the Lipid Droplet Counter, but others as well):
>
>
> http://imagejdocu.tudor.lu/doku.php?id=plugin:analysis:droplet_counter:start
>
> It's a bit of trial and error to decide which of these is the simplest one
> that works for you.
>
> -Jason
>
> -------
>
> Jason Miller, MD, PhD
>
> University of Michigan Kellogg Eye Center
>
>
> Home address:
>
> 117 Worden Ave
>
> Ann Arbor, MI 48103
>
> Cell: (415) 225-2134
>
> E-mail: *[hidden email] <[hidden email]
> >*
>
>
> "Forgiveness does not change the past, but it does enlarge the future." -
> Paul Boese
>
> On Thu, Jan 14, 2016 at 7:22 AM, Василий Попков <[hidden email]>
> wrote:
>
> > Good time of a day, friends.
> >
> > I have a z-stack of tmre colored mitochondria in a cell (red channel).
> I've
> > found an article, which is dealing with mitochondria morphology via
> > algorithms, but I can't really apply it - Im not good enough programmer
> > (well, I'm not at all).
> >
> > So. It is quite easy to detect mitochondria on each slice with some kind
> of
> > "analyze particles" or similar things. BUT. There are same mitochondria
> on
> > different slices. Are there any plugins which can understand that "this
> and
> > this are the same mitochondria as on previous slice, this is new one"?
> And
> > somehow to analyze it. Volume will be ideal, but at least something.
> >
> > Thank you a lot for you time. Any suggestions of what available plugins I
> > can use will be very helpful.
> >
> > Vasily
> >
> > Link to z-stack and article
> >
> https://www.dropbox.com/sh/af5hopz04ca8d73/AABIvB2SPf17I8h6rfNvbhS1a?dl=0
> >
> > --
> > ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> >
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>



--
Vasily A Popkov
Lab.Structure and function of mitochondria
A.N.Belozersky Institute of Physico-Chemical Biology
Moscow State University

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