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Re: Lenght measurement

Posted by Jan Eglinger on Jan 18, 2016; 12:01pm
URL: http://imagej.273.s1.nabble.com/Lenght-measurement-tp5015403p5015406.html

Hi Alexander,

the "longest shortest path" option of the Analyze Skeleton plugin might
do what you need:
http://imagej.net/AnalyzeSkeleton

You might need to use some blurring of your nucleus before segmenting
and skeletonizing it, to avoid over-skeletonization.

Hope that helps,
Jan



On 18.01.2016 12:16, Alexander V. Kalachev wrote:

> Dear Boris,
>
> thank you for fast reply. Under the term "length" I meant the length of a line running from the
> bottom to the top of nucleus and replicating nucleus' curvature (see attached image, black line).
>
> Alexander
>
> Mon, 18 Jan 2016 17:46:43 +0700
> Boris Golman <[hidden email]>
>
>> Alexander:
>>
>> If you like to measure the length of nucleus outline go to Analyze -> Set
>> measurements -> Perimeters and then Analyze --> Measure
>>
>> If you like to have an average measure of nucleus length look for Feret's
>> diameter frequently used for measurement of particle diameters.
>>
>> Anyway you need to have a definition of "length".
>>
>> Uspexov.
>>
>> Boris
>>
>> On Mon, Jan 18, 2016 at 5:19 PM, Alexander V. Kalachev <
>> [hidden email]> wrote:
>>
>>> Dear ImageJ users,
>>>
>>> for our current project we need to measure length of spermatozoa nucleus.
>>> It is curved in shape
>>> (see example image attached, nucleus outlined with white dashed line) and
>>> we have no idea how to
>>> measure its length. Could anyone provide us with an advice how to measure
>>> the length of such curved
>>> figure?
>>>
>>> With kind regards,
>>> Alexander
>>>
>>> --
>>> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>>>
>>
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>
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