Re: Lenght measurement

Posted by Jacqueline Ross on Jan 18, 2016; 11:34pm
URL: http://imagej.273.s1.nabble.com/Lenght-measurement-tp5015403p5015411.html

Hi Alexander,

Could you please post an image without any graphics/lines on it? It might be quite straightforward to try segmentation followed by skeletonization as Jan has suggested or someone may come up with another method that will work automatically but we can't really test out options with the image you sent:-).

Cheers,

Jacqui


Jacqueline Ross
Biomedical Imaging Microscopist
Biomedical Imaging Research Unit 
School of Medical Sciences 
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland 1142, NEW ZEALAND

Tel: [phone]
Fax: [phone]

http://www.fmhs.auckland.ac.nz/sms/biru/


-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Alexander V. Kalachev
Sent: Tuesday, 19 January 2016 12:17 a.m.
To: [hidden email]
Subject: Re: Lenght measurement

Dear Boris,

thank you for fast reply. Under the term "length" I meant the length of a line running from the bottom to the top of nucleus and replicating nucleus' curvature (see attached image, black line).

Alexander

Mon, 18 Jan 2016 17:46:43 +0700
Boris Golman <[hidden email]>

> Alexander:
>
> If you like to measure the length of nucleus outline go to Analyze ->
> Set measurements -> Perimeters and then Analyze --> Measure
>
> If you like to have an average measure of nucleus length look for
> Feret's diameter frequently used for measurement of particle diameters.
>
> Anyway you need to have a definition of "length".
>
> Uspexov.
>
> Boris
>
> On Mon, Jan 18, 2016 at 5:19 PM, Alexander V. Kalachev <
> [hidden email]> wrote:
>
> > Dear ImageJ users,
> >
> > for our current project we need to measure length of spermatozoa nucleus.
> > It is curved in shape
> > (see example image attached, nucleus outlined with white dashed
> > line) and we have no idea how to measure its length. Could anyone
> > provide us with an advice how to measure the length of such curved
> > figure?
> >
> > With kind regards,
> > Alexander
> >
> > --
> > ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> >
>
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