Re: Segmenting touching tubular objects

Posted by Gabriel Landini on Jan 30, 2016; 10:20am
URL: http://imagej.273.s1.nabble.com/Segmenting-touching-tubular-objects-tp5015488p5015490.html

On Friday 29 Jan 2016 16:47:26 ecophysulm wrote:
> I'd like to use ImageJ in an picture analysis for a in theory simple topic
> which appears not so easy for me in practise.
> I have photographs of small zylindric pellets and want to analyse them with
> the Particle analyzer. Beside the issue that the picture carries some
> reflections which I have more or less managed to get rid of. But the major
> issue appears to be the segmentation: the objects to analyse are not
> separated and are partly touching each other while having a elongated and
> cylindric form.

It will be difficult to separate 2 pellets side by side with the watershed
separation. It is not made for that kind of problem.

I see this as a problem that could be resolved outside the imaging part.
What you want is to have the objects completely separated from each other from
the start, so you do not have to separate them.

I would use some kind of surface or tray where the pellets fall/slot in a
preferred position and cannot overlap.
I am thinking of some surface with grooves or undulations?

Cheers
Gabriel

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