Re: measuring two lines perpendicularly

Posted by Gabriel Landini on Feb 02, 2016; 3:31pm
URL: http://imagej.273.s1.nabble.com/measuring-two-lines-perpendicularly-tp5015503p5015523.html

On Tuesday 02 Feb 2016 14:59:59 you wrote:
> I will try that plugin. It could be an effective solution for some spores
> associated with a rectangular ROI (elliptic spores especially). Thanks a
> lot.

Rather than doing it by hand, you could also try to binarise the spores, then
extract the feret diameter and the breadth (which is the maximum distances
from the feret to the boundaries, measured perpendicularly to the feret. Note
thought, that the segments on each side of the feret are not necessarily co-
linear. The Particles8 plugin can compute that.  Or compute the size of the
bounding box based in the rotating calipers algorithm. I think BoneJ can do
this.

Cheers

Gabriel

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