Re: merging IHC image from different slides

Posted by Saalfeld, Stephan on Feb 24, 2016; 10:34am
URL: http://imagej.273.s1.nabble.com/merging-IHC-image-from-different-slides-tp5015699p5015700.html

You can place the landmarks manually using the multi-point tool or try
automatic feature extraction:

http://fiji.sc/Feature_Extraction

Then, you can transform or warp the slices into a reference using this
plugin

http://fiji.sc/Landmark_Correspondences

Without seeing the images this is all I can tell.

Cheers,
Stephan



On Wed, 2016-02-24 at 00:53 -0800, Martino Bosco wrote:

> I have taken IHC images from different slides (of course, with
> different
> antibodies) using the same chromogen (DAB).
> How can I merge these image in one image in order to obtain a
> "virtual
> multicolour immunohistochemistry)? In particular, how can I use
> internal
> Landmarks to obtain a correct overlap between two or more image of
> the same
> histological field?
> Thanks for any help
>
> Martino Bosco, MD
> Anatomia Patologica
> Alba (CN), Italy
>
>
>
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