Posted by
Swayne, Theresa C. on
Mar 01, 2016; 2:42pm
URL: http://imagej.273.s1.nabble.com/changing-the-LUT-during-image-aquisition-tp5015756p5015758.html
Hi Ron,
To add to Herbie’s good advice, the “HiLo" LUT is the one you want.
Image > Color > Display LUTs gives you a montage of all the LUTs on your system, and you can download more from the ImageJ site and others.
If you are using Micro-Manager to capture, there was a thread on their mailing list that might help:
http://micro-manager.3463995.n2.nabble.com/Custom-LUT-in-Live-Window-tp7581866p7581888.htmlHope this helps,
Theresa
On Mar 1, 2016, at 8:37 AM, Ron Goldstein <
[hidden email]<mailto:
[hidden email]>> wrote:
Hi,
I would like to set the "default" 8-bit LUT of ImageJ so that 255 (saturated) is red and 0 (black) is blue during acquisition with digital monochrome cameras. this is standard on confocal microscopes, and is a big aid in obtaining images with as wide a dynamic range as possible.
In the LUT directory, there is no "grays" LUT, and changing the LUT only affects images, not acquisition windows.
I tried the live histogram plugin and the plot line profile, and they only seem to work on images and not on live acquisition windows.
Is this something that can be changed in ImageJ, or is it something that the camera software needs to control?
If it can be done within ImageJ, how is it done?
Thanks very much,
-Ron
Dr. Ron Goldstein
Bar-Ilan University
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Theresa Swayne, Ph.D.
Manager
Confocal and Specialized Microscopy Shared Resource<
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Herbert Irving Comprehensive Cancer Center
Columbia University Medical Center
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Phone: 212-851-4613
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