Re: macro threshold values
Posted by
CARL Philippe (LBP) on
URL: http://imagej.273.s1.nabble.com/macro-threshold-values-tp5015759p5015762.html
Dear Natalie,
I guess you are looking for a code like this:
nBins = 256;
ratio = 0.5;
run("Blobs (25K)");
getHistogram(values, counts, nBins);
Array.getStatistics(values, valuesMin, valuesMax, valuesMean, valuesStdDev);
setThreshold(0, ratio * valuesMax );
setOption("BlackBackground", true);
run("Convert to Mask");
with in your case ratio = 0.98;
My best regards.
Philippe
Philippe CARL
Laboratoire de Biophotonique et Pharmacologie
UMR 7213 CNRS - Université de Strasbourg
Faculté de Pharmacie
74 route du Rhin
67401 ILLKIRCH
Tel : +33(0)3 68 85 41 84
-----Message d'origine-----
De : ImageJ Interest Group [mailto:
[hidden email]] De la part de Natalie M
Envoyé : lundi 29 février 2016 18:43
À :
[hidden email]
Objet : macro threshold values
Hi all,
I am writing a macro to count cells in images I've taken of slides. Does anyone know what code I could use to have the macro threshold each image at the top 2% of each histogram, rather than at a set number value on each histogram?
Thanks in advance,
Natalie Morel
University of Wisconsin
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