http://imagej.273.s1.nabble.com/3d-segmentation-unit-vs-pixels-measures-tp5015529p5015788.html
> Hi Vasily,
>
> You can see voxels as cubes in the image space, so if you want to compute
> volumes in um^3, you count the number of voxels and multiply by the volume
> as one voxel as you mentioned. So in the case 2 you may find bigger volumes
> in um^3 with less voxels, because voxels in 2 are bigger that in 1. Hope
> this helps.
>
> Best,
>
> Thomas
>
>
> On 29/02/2016 20:30, Василий Попков wrote:
>
>> Hello.
>> I have the same problem for spot segmentation. pixel volume = unit volume.
>> Is there any way to understand what "the real" volume is, from pixel one?
>> Or to compare to images.
>>
>> For example if I have 2 objects on different images with 100 pixel volume
>> and raw images are
>> 7 pixels per µm
>> 1) Voxel size: 0.1428x0.1428x0.25 µm^3
>> 2) Voxel size: 0.1428x0.1428x0.3158 µm^3
>>
>> Can I count it somehow? Since voxels are not cubical I am a bit confosed.
>>
>> I guess I can say that V(1)/V(2) = (0.25)/(0.31) am I right?
>> Can I say, that theirs volume = v(pixel)*0.14*0.14*0.3185 or
>> v(pixel)*0.14*0.14*0.25?
>>
>> Thank you for clarifying it.
>>
>> 2016-02-11 10:39 GMT+03:00 Thomas Boudier <
[hidden email]>:
>>
>> Hi,
>>>
>>> The "simple segmentation" plugin does not copy the calibration properties
>>> from the raw image to the segmented image, this bug will be fixed in a
>>> next
>>> update.
>>>
>>> Best,
>>>
>>> Thomas
>>>
>>>
>>> On 03/02/2016 20:07, Василий Попков wrote:
>>>
>>> Good time of a day. I am using 3d segmentation package (
>>>>
http://imagejdocu.tudor.lu/doku.php?id=plugin:stacks:3d_ij_suite:start>>>> ).
>>>> And I have exactly the same values for pixels volumes and unity volumes
>>>> for
>>>> each image (in output), which is obviously not true. There is
>>>> information
>>>> about voxel size in image info, but it seems it is not used.
>>>>
>>>> Anyone can give me a tip how I can make it to give me correct values in
>>>> mkm?
>>>>
>>>>
>>>> --
>>> /***************************************************************/
>>> Thomas Boudier, Associate Professor, UPMC,
>>> Université Pierre et Marie Curie, Paris, France.
>>> BioInformatics Institute (BII)/IPAL, Singapore.
>>> /**************************************************************/
>>>
>>> --
>>> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html>>>
>>>
>>
>>
> --
> /***************************************************************/
> Thomas Boudier, Associate Professor, UPMC,
> Université Pierre et Marie Curie, Paris, France.
> BioInformatics Institute (BII)/IPAL, Singapore.
> /**************************************************************/
>
> --
> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html>