Posted by
Swayne, Theresa C. on
Mar 07, 2016; 3:58pm
URL: http://imagej.273.s1.nabble.com/multimeasure-problem-tp5015810p5015816.html
Hi Jeremy,
Is the data perhaps being read as a composite image (stack of channels with a C next to the slice selector)? MultiMeasure will only measure only the current channel of a composite image, even though other “stack” commands (e.g. Set Slice…) will interpret each channel as a “slice.”
If this is the case, you can run Stack to Hyperstack and re-configure the stack as a z stack (swap the numbers appearing in the Channels and Slices boxes).
If that doesn’t solve the problem, it might be helpful to post the problem stack somewhere.
Hope this helps,
Theresa
On Mar 6, 2016, at 2:41 PM, Jeremy Adler <
[hidden email]<mailto:
[hidden email]>> wrote:
When I open a stack with 32 images and run multi measure I get one line of measurements, not the 32 I expected.
The problem seems to be that ImageJ does not recognize that the image is a stack - nSlices correctly reports the number of images in the stack and I can run through the stack manually but Show Info reports a bit depth of only 1.
If the stack is broken down into images (stack to images) and reformed (images to stack) then Show Info correctly reports the depth as 32 and multi measure runs properly.
The stack came from micromanager and I am running version 1.50g
any suggestions.
--
ImageJ mailing list:
http://imagej.nih.gov/ij/list.html------------------------------------
Theresa Swayne, Ph.D.
Manager
Confocal and Specialized Microscopy Shared Resource<
http://hiccc.columbia.edu/research/sharedresources/confocal>
Herbert Irving Comprehensive Cancer Center
Columbia University Medical Center
1130 St. Nicholas Ave., Room 222A
New York, NY 10032
Phone: 212-851-4613
[hidden email]<mailto:
[hidden email]>
--
ImageJ mailing list:
http://imagej.nih.gov/ij/list.html