Re: color doppler ultrasound quantification

Posted by albertocontri on Apr 03, 2016; 12:24pm
URL: http://imagej.273.s1.nabble.com/color-doppler-ultrasound-quantification-tp5007582p5016049.html

Dear dr. Straatman,

I'm sorry because I acknowledged you only now for your response.

I shelved the project until now, but now it is the time for me to complete the work.

You suggested me a way to quantify the blue and red areas in my image. It is important to say that I don't need to separate the measurement of the blue and red areas, but them could be merged.

I try to explain better what the ImageJ should give me. I need to quantify the proportion of the blue+red areas compared with the area enclosed in the ROI.

I send you another image where I created around the region of interest (ROI) a perimeter with the freehand selections function. Now, I need to calculate the proportion(ratio) between the blue + red areas and the area enclosed in the yellow line. I could also calculate the ratio in a second time, but I need to have both the areas (total inside the yellow line and blue+red areas).

I'm able to measure the area inside the yellow line but I'm not able to keep on.

I hope you could suggest me how I can complete the analysis.

Kind regards and thanks a lot for your time.

Alberto Contri

2014-05-06 11:30 GMT+02:00 Straatman, Kees (Dr.) [via ImageJ] <[hidden email]>:

Dear Alberto,

Try something like color > split channels. Select the individual red and blue channels and run on each a threshold (Image > Adjust > threshold) with settings that select the areas you are interested in. Under Analyse > Set Measurements select Area and Area fraction and run Analyse > Analyse Particles for both your red and blue image. Hope this gives the result you are after.

Best wishes

Kees

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of albertocontri
Sent: 05 May 2014 23:19
To: [hidden email]
Subject: color doppler ultrasound quantification

Hallo everyone,
I'm new of the forum and of ImageJ. Now, I ask a question that have probably an obvious answer.

I need to quantify the amount of red and blue pixels in an image, and the proportion of the red and blue areas compared to the whole gray image. The image that I need to analyze is a color doppler image, in which the colours represented the blood vessels. I need to quantify the proportion of the boold vessels in the organ that I examine. If need, I could attach an example.

I hope one of you could help a wretched researcher...

Thanks in advance

Alberto



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