Hi Alberto,
if you have selected 'Area%' in 'Set Measurements', the %Area column
gives you the area fraction of the thresholded area (if there is a
threshold) or the area with non-zero pixels (if there is no threshold).
If you have a binary image with an inverting LUT (as in your case), the
black area is non-zero (255), the background is zero.
If there is an area selection (=region of interest), the area fraction
is for the region inside the selection, ignoring everything else. You
can easily try this by selecting a region that contains only black or
only white pixels; you will get 100 or 0%, respectively.
Michael
________________________________________________________________
On 2016-04-04 09:09, albertocontri wrote:--> [hidden email]>:
> Dear Michael,
> thank you for all your explanations.
> I'm near the solution of my troubles. I send you the point where I am: the
> colored parts are black, the gray one are white.
> Now, within the yellow line, I need to have the proportion between the
> black area and the area delimited within the yellow line. I tried with the
> measure function, and the result table gave me the total area enclosed in
> the yellow line and a percent, but I have not understood to what the
> percent referred. Maybe it is more simple if the result table give me bith
> the areas. I need to limit the evaluation to the region enclosed in the
> yellow line, because it is the structure of my interest.
>
> Thanks a lot
>
> Alberto
>
>
> 2016-04-03 19:46 GMT+02:00 Michael Schmid-3 [via ImageJ] <>>> [hidden email] <http:///user/SendEmail.jtp?type=node&node=5016050&i=0>>:>
>> Hi Alberto,
>>
>> if you just need to distinguish between gray and colored areas, you can
>> simply transform the image to an HSB stack (hue/saturation/brightness) and
>> threshold the S (saturation) channel. The saturation value is zero for
>> gray pixels.
>> The small number in the Threshold panel will tell you the fraction of the
>> red area in percent while moving the Threshold sliders.
>> For a list output (Results Table), use 'Measure' with 'Area Fraction'
>> enabled in 'Analyze>Set Measurements'.
>>
>> Michael
>> __________________________________________________________
>>
>> On Sun, April 3, 2016 14:24, albertocontri wrote:
>>
>>> Dear dr. Straatman,
>>>
>>> I'm sorry because I acknowledged you only now for your response.
>>> I shelved the project until now, but now it is the time for me to
>> complete
>>> the work.
>>> You suggested me a way to quantify the blue and red areas in my image.
>> It
>>> is important to say that I don't need to separate the measurement of the
>>> blue and red areas, but them could be merged.
>>> I try to explain better what the ImageJ should give me. I need to
>> quantify
>>> the proportion of the blue+red areas compared with the area enclosed in
>>> the
>>> ROI.
>>> I send you another image where I created around the region of interest
>>> (ROI) a perimeter with the freehand selections function. Now, I need to
>>> calculate the proportion(ratio) between the blue + red areas and the
>> area
>>> enclosed in the yellow line. I could also calculate the ratio in a
>> second
>>> time, but I need to have both the areas (total inside the yellow line
>> and
>>> blue+red areas).
>>> I'm able to measure the area inside the yellow line but I'm not able to
>>> keep on.
>>>
>>> I hope you could suggest me how I can complete the analysis.
>>>
>>>
>>> Kind regards and thanks a lot for your time.
>>>
>>> Alberto Contri
>>>
>>> 2014-05-06 11:30 GMT+02:00 Straatman, Kees (Dr.) [via ImageJ] <>> http://imagej.1557.x6.nabble.com/color-doppler-ultrasound-quantification-tp5007582p5016050.html>>
>>>
>>>> Dear Alberto,
>>>>
>>>> Try something like color > split channels. Select the individual red
>> and
>>>> blue channels and run on each a threshold (Image > Adjust > threshold)
>>>> with
>>>> settings that select the areas you are interested in. Under Analyse >
>>>> Set
>>>> Measurements select Area and Area fraction and run Analyse > Analyse
>>>> Particles for both your red and blue image. Hope this gives the result
>>>> you
>>>> are after.
>>>>
>>>> Best wishes
>>>>
>>>> Kees
>>>>
>>>> -----Original Message-----
>>>> From: ImageJ Interest Group [mailto:[hidden email]
>>>> <http:///user/SendEmail.jtp?type=node&node=5007592&i=0>] On Behalf Of
>>>> albertocontri
>>>> Sent: 05 May 2014 23:19
>>>> To: [hidden email]
>>>> <http:///user/SendEmail.jtp?type=node&node=5007592&i=1>
>>>> Subject: color doppler ultrasound quantification
>>>>
>>>> Hallo everyone,
>>>> I'm new of the forum and of ImageJ. Now, I ask a question that have
>>>> probably an obvious answer.
>>>>
>>>> I need to quantify the amount of red and blue pixels in an image, and
>>>> the
>>>> proportion of the red and blue areas compared to the whole gray image.
>>>> The
>>>> image that I need to analyze is a color doppler image, in which the
>>>> colours
>>>> represented the blood vessels. I need to quantify the proportion of the
>>>> boold vessels in the organ that I examine. If need, I could attach an
>>>> example.
>>>>
>>>> I hope one of you could help a wretched researcher...
>>>>
>>>> Thanks in advance
>>>>
>>>> Alberto
>>>>
>>>>
>>>>
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>>>>
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