Hi Alberto,
make sure the spatial calibration of the image is correct:
http://imagej.net/SpatialCalibration
http://serc.carleton.edu/eyesinthesky2/week2/get_to_know_imagej.html
Then check 'Area' in 'Set Measurements' and you will get the area of the
selection in the units used for calibrating the image.
http://rsb.info.nih.gov/ij/docs/guide/146-30.html#toc-Subsection-30.7
Multiply the area and the percentage/100 to get the area.
Alternatively, threshold the area of interest and check 'Limit to
threshold' in the Measurements options. Then you get the thresholded
area (but not the percentage).
Michael
________________________________________________________________
On 2016-04-05 09:34, albertocontri wrote:--
> Dear Michael, thanks a lot. I've reach my objective.
> Finally, do you think I could have the crude values of the areas (in the
> ROI and colored) instead the percentage?
>
> Thanks a lot for everything and I hope to stop my questions.
>
> Alberto Contri
>
> 2016-04-04 9:46 GMT+02:00 Michael Schmid-3 [via ImageJ] <>>> [hidden email] <http:///user/SendEmail.jtp?type=node&node=5016054&i=0>>:> [hidden email]>:
>
>> Hi Alberto,
>>
>> if you have selected 'Area%' in 'Set Measurements', the %Area column
>> gives you the area fraction of the thresholded area (if there is a
>> threshold) or the area with non-zero pixels (if there is no threshold).
>> If you have a binary image with an inverting LUT (as in your case), the
>> black area is non-zero (255), the background is zero.
>>
>> If there is an area selection (=region of interest), the area fraction
>> is for the region inside the selection, ignoring everything else. You
>> can easily try this by selecting a region that contains only black or
>> only white pixels; you will get 100 or 0%, respectively.
>>
>> Michael
>> ________________________________________________________________
>>
>> On 2016-04-04 09:09, albertocontri wrote:
>>
>>> Dear Michael,
>>> thank you for all your explanations.
>>> I'm near the solution of my troubles. I send you the point where I am:
>> the
>>> colored parts are black, the gray one are white.
>>> Now, within the yellow line, I need to have the proportion between the
>>> black area and the area delimited within the yellow line. I tried with
>> the
>>> measure function, and the result table gave me the total area enclosed
>> in
>>> the yellow line and a percent, but I have not understood to what the
>>> percent referred. Maybe it is more simple if the result table give me
>> bith
>>> the areas. I need to limit the evaluation to the region enclosed in the
>>> yellow line, because it is the structure of my interest.
>>>
>>> Thanks a lot
>>>
>>> Alberto
>>>
>>>
>>> 2016-04-03 19:46 GMT+02:00 Michael Schmid-3 [via ImageJ] <>> http://imagej.1557.x6.nabble.com/color-doppler-ultrasound-quantification-tp5007582p5016054.html>>
>>>
>>>> Hi Alberto,
>>>>
>>>> if you just need to distinguish between gray and colored areas, you can
>>>> simply transform the image to an HSB stack (hue/saturation/brightness)
>> and
>>>> threshold the S (saturation) channel. The saturation value is zero for
>>>> gray pixels.
>>>> The small number in the Threshold panel will tell you the fraction of
>> the
>>>> red area in percent while moving the Threshold sliders.
>>>> For a list output (Results Table), use 'Measure' with 'Area Fraction'
>>>> enabled in 'Analyze>Set Measurements'.
>>>>
>>>> Michael
>>>> __________________________________________________________
>>>>
>>>> On Sun, April 3, 2016 14:24, albertocontri wrote:
>>>>
>>>>> Dear dr. Straatman,
>>>>>
>>>>> I'm sorry because I acknowledged you only now for your response.
>>>>> I shelved the project until now, but now it is the time for me to
>>>> complete
>>>>> the work.
>>>>> You suggested me a way to quantify the blue and red areas in my image.
>>>> It
>>>>> is important to say that I don't need to separate the measurement of
>> the
>>>>> blue and red areas, but them could be merged.
>>>>> I try to explain better what the ImageJ should give me. I need to
>>>> quantify
>>>>> the proportion of the blue+red areas compared with the area enclosed
>> in
>>>>> the
>>>>> ROI.
>>>>> I send you another image where I created around the region of interest
>>>>> (ROI) a perimeter with the freehand selections function. Now, I need
>> to
>>>>> calculate the proportion(ratio) between the blue + red areas and the
>>>> area
>>>>> enclosed in the yellow line. I could also calculate the ratio in a
>>>> second
>>>>> time, but I need to have both the areas (total inside the yellow line
>>>> and
>>>>> blue+red areas).
>>>>> I'm able to measure the area inside the yellow line but I'm not able
>> to
>>>>> keep on.
>>>>>
>>>>> I hope you could suggest me how I can complete the analysis.
>>>>>
>>>>>
>>>>> Kind regards and thanks a lot for your time.
>>>>>
>>>>> Alberto Contri
>>>>>
>>>>> 2014-05-06 11:30 GMT+02:00 Straatman, Kees (Dr.) [via ImageJ] <
>>>>> [hidden email] <http:///user/SendEmail.jtp?type=node&node=5016050&i=0>>:
>>
>>>>
>>>>>
>>>>>> Dear Alberto,
>>>>>>
>>>>>> Try something like color > split channels. Select the individual red
>>>> and
>>>>>> blue channels and run on each a threshold (Image > Adjust >
>> threshold)
>>>>>> with
>>>>>> settings that select the areas you are interested in. Under Analyse >
>>>>>> Set
>>>>>> Measurements select Area and Area fraction and run Analyse > Analyse
>>>>>> Particles for both your red and blue image. Hope this gives the
>> result
>>>>>> you
>>>>>> are after.
>>>>>>
>>>>>> Best wishes
>>>>>>
>>>>>> Kees
>>>>>>
>>>>>> -----Original Message-----
>>>>>> From: ImageJ Interest Group [mailto:[hidden email]
>>>>>> <http:///user/SendEmail.jtp?type=node&node=5007592&i=0>] On Behalf
>> Of
>>>>>> albertocontri
>>>>>> Sent: 05 May 2014 23:19
>>>>>> To: [hidden email]
>>>>>> <http:///user/SendEmail.jtp?type=node&node=5007592&i=1>
>>>>>> Subject: color doppler ultrasound quantification
>>>>>>
>>>>>> Hallo everyone,
>>>>>> I'm new of the forum and of ImageJ. Now, I ask a question that have
>>>>>> probably an obvious answer.
>>>>>>
>>>>>> I need to quantify the amount of red and blue pixels in an image, and
>>>>>> the
>>>>>> proportion of the red and blue areas compared to the whole gray
>> image.
>>>>>> The
>>>>>> image that I need to analyze is a color doppler image, in which the
>>>>>> colours
>>>>>> represented the blood vessels. I need to quantify the proportion of
>> the
>>>>>> boold vessels in the organ that I examine. If need, I could attach an
>>>>>> example.
>>>>>>
>>>>>> I hope one of you could help a wretched researcher...
>>>>>>
>>>>>> Thanks in advance
>>>>>>
>>>>>> Alberto
>>>>>>
>>>>>>
>>>>>>
>>>>>> --
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