Re: combine data from multiple profile measurements of a time series to single data sheet

Posted by Kota Miura on Apr 13, 2016; 10:03pm
URL: http://imagej.273.s1.nabble.com/combine-data-from-multiple-profile-measurements-of-a-time-series-to-single-data-sheet-tp5016124p5016128.html

Hi,

You don't really need a script for that.

1. Reslice with "100 slices", then you get a stack of 100 slices (= width
of the line ROI)

2. Do Z-Projection. If you want an average for each x position, then choose
"average" as the projection method. This then gives you a single projected
2D image.

3. Save this image as a text image. It will then be a text file with
average intensity profile per line (= time point)

good luck,
Kota

On Wed, Apr 13, 2016 at 7:52 PM, cpanders <[hidden email]>
wrote:

> I have a large time series (thousands of frames) of micrographs of a
> stationary structure that pulsates over time.  I need to collect data from
> a
> single profile measurement (of a 100 pixel wide x ca. 4mm long
> perpendicular
> line) of each time point (frame) and combine into a single data sheet.
>
> This problem must have been solved previously but I could find no suitably
> applicable posts.  I have used the ROI manager and multi measure to analyze
> the same  images for other parameters but do not see a way to apply these
> tools to the "plot profile" measurement.
>
>  Any formulas/scripts/macros that can be sent will be so gratefully
> appreciated!
>
>
>
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> Sent from the ImageJ mailing list archive at Nabble.com.
>
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Dr. Kota Miura <[hidden email]>

Freelance Image Analyst
EMBL
Meyerhofstr. 1, 69117 Heidelberg, GERMANY
Tel [phone]
Mobile [phone]http://cmci.embl.de
++++++++++++++++++

Dr. Kota Miura  <[hidden email]>
Research Collaboration Fellow
National Institute of Basic Biology
Okazaki, 444-8585
JAPAN
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