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Re: OME Tiffs and macros

Posted by Roger Leigh-2 on Apr 20, 2016; 1:32pm
URL: http://imagej.273.s1.nabble.com/OME-Tiffs-and-macros-tp5016081p5016195.html

On 08/04/2016 01:52, nellypledge wrote:
> Hi all.
>
> It appears I've created some ome.tifs using micro-manager (MM) that will
> open when I drag and drop, but not in a macro.  It tells me that the file is
> not a supported format when I try to open it from a macro loop.

> Thanks in advance for your time.  Any pointers would be much appreciated.

> /[WARNING] Opening was canceled.
> [INFO] Reading IFDs
> [INFO] Populating metadata
> [INFO] Checking comment style
> java.io.IOException: io.scif.img.ImgIOException: java.io.IOException:
> io.scif.FormatException: Invalid TIFF file
>       at
> io.scif.services.DefaultDatasetIOService.open(DefaultDatasetIOService.java:134)
>       at
> io.scif.services.DefaultDatasetIOService.open(DefaultDatasetIOService.java:109)

It looks like ImageJ is using scifio to open the file, rather than
Bio-Formats.  I would suggest turning this off and retrying
(Edit->Options->ImageJ2) to see if this makes any difference.

If you still have problems, please do let us know.


Kind regards,
Roger Leigh

--
Dr Roger Leigh -- Open Microscopy Environment
Wellcome Trust Centre for Gene Regulation and Expression,
School of Life Sciences, University of Dundee, Dow Street,
Dundee DD1 5EH Scotland UK   Tel: (01382) 386364

The University of Dundee is a registered Scottish Charity, No: SC015096

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