Help Needed for Oil Droplet Identification

Posted by MembraneSci on Apr 21, 2016; 9:03pm
URL: http://imagej.273.s1.nabble.com/Help-Needed-for-Oil-Droplet-Identification-tp5016212.html

Hi folks,

I'm looking for help on identifying the droplets in three photos (below signature). I've tried many different combinations of commands (subtract background, threshold, gaussian blur, find edges, sharpen, enhance contrast, make binary, and watershed), but I just can't seem to get a good enough threshold image for accurate particle sizing.

I would be so appreciative to see a list of commands that can get me a good image!

Thanks for your help,
Leo