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Re: Counting cells using Image J

Posted by Christian Renicke on Apr 27, 2016; 4:05pm
URL: http://imagej.273.s1.nabble.com/Re-Counting-cells-using-Image-J-tp5016253p5016255.html

Hi Bridget,

At what point do you encounter the problems with cell counter? This
question may be stupid, but did you initialize your image for counting?

Best,

Chris

Christian Renicke, M.Sc.
Ph.D. Student
University of Marburg - Department of Biology
Group of Prof. Dr. H.U. Mösch - Junior Group of Dr. C. Taxis
Karl-von-Frisch Str. 8
D-35043 Marburg

Phone: +49 6421 / 2823246
E-Mail: [hidden email]

Am 27.04.2016 um 17:09 schrieb Fomenky, Bridget:

> Hi everyone,
>
> I will like  to count cells from Alcian blue staining using image J. I have tried following the steps plugin   >analyse> cell counter and it does not work.
> Could anyone tell me what to do?
>
> Thanks
>
> Bridget
>
>
> -----Original Message-----
> From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Oceane
> Sent: April-27-16 10:19 AM
> To: [hidden email]
> Subject: Counting structures within measured ROI
>
> Dear all,
> I am new to Image J and and I am slowly starting to realize the levels of complexity it offers.
> I want to measure several ROIs in my picture, and manually count cells only within those who have a minor/major axis ratio >0.85.
> I tried using the freehand selection tool and measurement command, but I can't seem to link the ROIs to the measurement, select the ones that are round enough, and then link my cell count to one particular ROI.
> Is there a way for me to produce a single table containing the ROI measurements and the structure count ?
> Thanks a LOT for your help!
> Océane
>
>
>
> --
> View this message in context: http://imagej.1557.x6.nabble.com/Counting-structures-within-measured-ROI-tp5016248.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
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