Posted by
Fomenky, Bridget on
Apr 27, 2016; 8:38pm
URL: http://imagej.273.s1.nabble.com/Re-Counting-cells-using-Image-J-tp5016253p5016261.html
Hi Mike,
The cells are individually visible. I am new to image J I do not know what you mean by threshold of the cells .
Thanks
Bridget
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Mike Esterman
Sent: April-27-16 2:36 PM
To:
[hidden email]
Subject: Re: Counting cells using Image J
Two important things you have not mention, are the cells individually visible, that is are they all separated by some background, at least a couple of pixels. If not you will need to process with a watershed, or an open or close operation. Secondly you didn't mention of you thresholded your cells, that have to sufficiently distinct from background so that you end up with a binary image - cells foreground, everything else background.
Mike
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Fomenky, Bridget
Sent: Wednesday, April 27, 2016 11:10 AM
To:
[hidden email]
Subject: Re: Counting cells using Image J
Hi everyone,
I will like to count cells from Alcian blue staining using image J. I have tried following the steps plugin >analyse> cell counter and it does not work.
Could anyone tell me what to do?
Thanks
Bridget
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Oceane
Sent: April-27-16 10:19 AM
To:
[hidden email]
Subject: Counting structures within measured ROI
Dear all,
I am new to Image J and and I am slowly starting to realize the levels of complexity it offers.
I want to measure several ROIs in my picture, and manually count cells only within those who have a minor/major axis ratio >0.85.
I tried using the freehand selection tool and measurement command, but I can't seem to link the ROIs to the measurement, select the ones that are round enough, and then link my cell count to one particular ROI.
Is there a way for me to produce a single table containing the ROI measurements and the structure count ?
Thanks a LOT for your help!
Océane
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