Re: Segmenting Circular Objects that are touching multiple other objects
Posted by Albert Cardona-2 on Apr 30, 2016; 4:59pm URL: http://imagej.273.s1.nabble.com/Segmenting-Circular-Objects-that-are-touching-multiple-other-objects-tp5016288p5016292.html
I would approach this with a machine learning approach, like the Trainable Segmentation plugin in Fiji.
Albert
> On Apr 29, 2016, at 5:21 PM, smithd10 <[hidden email]> wrote:
>
> Hello,
>
> I am attempting to size microspheres that I have imaged on TEM. The
> microspheres are touching multiple other microspheres and I am having
> difficulty segmenting/thresholding the objects to maintain the original size
> of the microsphere. I either have the problem of the negative space becoming
> the main focus of the particle analysis, or I have thresholded the image so
> much that the particle size is no longer accurately measured.
>
> In other programs, there is the ability to automatically draw circles around
> an object based on the circularity of part of that that object. Is that
> ability available in ImageJ?
>
> Thank you.
>
> <http://imagej.1557.x6.nabble.com/file/n5016288/Pic13.gif>
>
>
>
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