http://imagej.273.s1.nabble.com/Mutliview-Deconvolution-PSF-Format-tp5016323p5016327.html
it should be 3d Tiff with the same calibration as the input data. It will apply the same transformation to the PSF(s) as to each respective stack that us deconvoled.
> On May 5, 2016, at 23:36, Pavel Tomancak <
[hidden email]> wrote:
>
>
> Begin forwarded message:
>
>> From: "Anthony, Neil" <
[hidden email]>
>> Subject: Mutliview Deconvolution PSF Format
>> Date: May 5, 2016 7:53:45 PM GMT+02:00
>> To:
[hidden email]
>> Reply-To:
[hidden email]
>>
>> Hi all,
>> Can anybody please advise on the correct format for the PSF that can be provided to the multiview reconstruction deconvolution plugin by Stephan Preibisch.
>> Also, how does the pixel size of that PSF change things? Both if I let it find them from the reference beads and if I provide a PSF (assumed) zstack.
>>
>> Thanks
>> Neil
>>
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