setThreshold Macro command for fluorescent microscopy

Posted by Morange on
URL: http://imagej.273.s1.nabble.com/setThreshold-Macro-command-for-fluorescent-microscopy-tp5016342.html

Hello,

I am trying to reduce the threshold of multiple microscope images by using the following script:

run("Invert");
run("8-bit");
setThreshold(0, 162, "black & white");
run("Measure");

however, the function only converts the images to black and white and have not adjusted the threshold. How can I change the code to set my desired threshold?

Edit: another problem that I had was some of the images were inverted again additional to the first "Invert" after the setThreshold has been called.