Posted by
nellypledge on
URL: http://imagej.273.s1.nabble.com/Mutliview-Deconvolution-PSF-Format-tp5016323p5016349.html
Thanks Stephan.
Am I correct in saying it's a a stack from a single view?
Also, can you advise on the pixel dimensions (e.g. 7x7x11 vs 15x15x9) both for user calibrated and auto generated?
Thanks
Neil
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Subject: Fwd: Mutliview Deconvolution PSF Format
Hi Anthony,
it should be 3d Tiff with the same calibration as the input data. It will apply the same transformation to the PSF(s) as to each respective stack that us deconvoled.
Hope this helps,
Stephan
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Subject: Fwd: Mutliview Deconvolution PSF Format
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Subject: Mutliview Deconvolution PSF Format
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Hi all,
Can anybody please advise on the correct format for the PSF that can be provided to the multiview reconstruction deconvolution plugin by Stephan Preibisch.
Also, how does the pixel size of that PSF change things? Both if I let it find them from the reference beads and if I provide a PSF (assumed) zstack.
Thanks
Neil
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