http://imagej.273.s1.nabble.com/Is-it-possible-to-measure-with-sub-pixel-resolution-tp5016356p5016359.html
To obtain subpixel resolution apply first deconvolution.
> Dear list members,
>
> I was wondering if there's a specific option/way to do measurements in an
> image with sub-pixel resolution?
>
> I paste below macro code to illustrate what I mean. The following creates a
> 10x10 pixels image, half of which is back and the other half white. If I
> draw a triangle that is half in the black and half in the white area, with
> a total area of 1pixel, I get a mean value of 255, but I would have
> expected 127.5
>
> newImage("Untitled", "8-bit black", 10, 10, 1);
>
> makeRectangle(5, 0, 5, 10);
>
> setBackgroundColor(255, 255, 255);
>
> run("Clear", "slice");
>
> makePolygon(4,5,6,5,5,6);
>
> run("Measure");
>
>
> Does anyone know if it's possible to get 127.5?
>
> Thanks for your help,
>
> Best wishes,
>
> Emmanuel
>
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