Automation Stack Processing
Posted by Aedifex on May 09, 2016; 11:38am
URL: http://imagej.273.s1.nabble.com/Automation-Stack-Processing-tp5016362.html
Hi Im currently working on a long term observation setup for stem cells which generates big image sequences in an interval defined by the user. Usually I end up with somewhat around 1200 pictures, my problem is that im measuring both bright field and fluorescence and that i need to overlay the fluorescence images with the corresponding bright field measurement at 50% opacity (the first image is always bright, the second fluorescence). I was wondering if it would be possible to automate this work with a macro in imagej but im biologist and not very good at programming. It would be great if someone could help me out with that.
URL: http://imagej.273.s1.nabble.com/Automation-Stack-Processing-tp5016362.html
Hi Im currently working on a long term observation setup for stem cells which generates big image sequences in an interval defined by the user. Usually I end up with somewhat around 1200 pictures, my problem is that im measuring both bright field and fluorescence and that i need to overlay the fluorescence images with the corresponding bright field measurement at 50% opacity (the first image is always bright, the second fluorescence). I was wondering if it would be possible to automate this work with a macro in imagej but im biologist and not very good at programming. It would be great if someone could help me out with that.
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